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SPOT synthesis will not be restricted to proteinogenic amino acids, [lawyers in my area](https://gitea.test.ansende.com/tatianadhakiya) several commercially out there modified amino acids additionally can be utilized for synthesis, allowing peptides to be modified before and after the final cleavage of the side-chain protection group and, furthermore, it additionally allows incorporation of phosphorylated, methylated or acetylated amino acids eleven. |